Efficiency of Microorganisms and Effectiveness of Biodegradation Techniques on LDPE Plastics: A Systematic Review

Introduction The aim of the research was to demonstrate the efficiency of microorganisms and the effectiveness of biodegradation techniques on Low-density polyethylene (LDPE) plastics. The research question was: What is the efficiency of LDPE-degrading microorganisms and the effectiveness of biodegradation techniques? Methods The systematic review was based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Articles were obtained from Scopus, Web of Science (WOS), Embase, and Google Scholar. The DeCS/Mesh search terms were: Low-density polyethylene, efficiency, biodegradation, microbial consortia, fungi, bacteria. Inclusion criteria were: scientific articles that included bacteria, fungi, and microbial consortia reported as LDPE degraders that report the percentage of weight loss; articles published from January 2010 to October 2022, and publications in Spanish and English with open access. Exclusion criteria were: studies that do not report gravimetry, the biodegradation time of LDPE, and the genus or species of the polyethylene-degrading microorganism. Results Out of 483 studies found, 50 were included in this Systematic Review (SR). The most frequent study techniques were scanning electron microscopy (SEM), gravimetry, and fourier transform infrared spectroscopy (FTIR), and in the case of microorganisms, the most studied belonged to the genus Pseudomonas, Bacillus, and Aspergillus. Regarding the isolation place, the most frequent mentioned in the reviewed articles were landfill soil and sanitary landfill soil. The efficiency of LDPE-degrading microorganisms was higher in bacteria such as Enterobacter spp., Pantoea spp., Pseudomonas spp., Escherichia coli, and Bacillus spp., which obtained a range of DE of 9.00-70.00%, 24.00-64%, 1.15 – 61.00%, 45.00%, and 1.5-40% with DT of 4-150, 120, 4-150, 30, and 30-120 days, respectively; in the case of fungi, the main microorganisms are Neopestalotiopsis phangngaensis, Colletotrichum fructicola, and Thyrostroma jaczewskii with efficiencies of 54.34, 48.78, and 46.34%, in 90 days, respectively; and the most efficient microbial consortia were from Enterobacter spp. and Pantoea sp. with 38.00 – 81.00%, in 120 days; and, Pseudomonas protegens, Stenotrophomonas sp., B. vallismortis and Paenibacillus sp. with 55. 00 – 75.00% in 120 days. Conclusions The most efficient microorganisms in LDPE degradation are Enterobacter spp., Pantoea spp., Pseudomonas spp., Escherichia coli, and Bacillus spp.; in fungi Neopestalotiopsis phangngaensis, Colletotrichum fructicola, and Thyrostroma jaczewskii; and in microbial consortia, those formed by Enterobacter spp. and Pantoea sp., and that of P. protegens, Stenotrophomonas sp., B. vallismortis and Paenibacillus sp.; and the most effective techniques used in LDPE biodegradation are SEM, gravimetry, and FTIR.


Introduction
The aim of the research was to demonstrate the efficiency of microorganisms and the effectiveness of biodegradation techniques on Low-density polyethylene (LDPE) plastics.The research question was: What is the efficiency of LDPE-degrading microorganisms and the effectiveness of biodegradation techniques?

Methods
The systematic review was based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement.Articles were obtained from Scopus, Web of Science (WOS), Embase, and Google Scholar.The DeCS/Mesh search terms were: Low-density polyethylene, efficiency, biodegradation, microbial consortia, fungi, bacteria.Inclusion criteria were: scientific articles that included bacteria, fungi, and microbial consortia reported as LDPE degraders that report the percentage of weight loss; articles published from January 2010 to October 2022, and publications in Spanish and English with open access.Exclusion criteria were: studies that do not report
Any reports and responses or comments on the article can be found at the end of the article.

Introduction
Plastics are synthetic polymeric molecules characterized by their versatility, lightness, low cost, and high durability. 1 Among the most common are polypropylene, polyethylene, nylon, and polycarbonate, which are considered highly persistent with a capacity for bioaccumulation; they also contaminate the soil, mainly cultivable areas, thus reducing the water filtration capacity and fertilization of plants. 2,3Currently, they have become one of the most significant pollutants in marine ecosystems where most of these float and disintegrate into small fragments when exposed to the sun, taking the name of microplastics. 4 Plastics are classified into 1 : easily degradable, which includes biologically phase plastics such as compostable and biodegradable ones, and 2 difficultly degradable, among which thermoset and thermoplastic plastics such as polypropylene (PP), polyvinyl chloride (PVC), polystyrene (PS), polytetrafluoroethylene (PTFE), and low-density polyethylene (LDPE) can be mentioned. 5The latter, globally, are the most marketed, with a production of 390.7 million tons in 2021, of which 50% was produced in Asia and 22% in America. 6Polyethylene is the most commonly used plastic in everyday life, accounting for 96% of all plastics on the market. 7PE accounts for 64%, and is primarily used in the form of bags, wrappings, and containers, which are discarded after use. 8The mismanagement of plastic waste increases daily, mainly in Asian countries such as China, Indonesia, the Philippines, Vietnam, Malaysia, Thailand, and in Western countries like the United States.Of the plastics produced, considered to be 18 billion metric tons, 6% are incinerated, 23% are reused, 62% are disposed of in landfills, and 9% are considered recycled. 1 has been demonstrated in-vitro that the ingestion of plastics by living beings produces a high impact on fauna.It is mentioned that they cause neurotoxic and degenerative damages in rodents, marine invertebrates, fish, and mammals, who are exposed to the presence of high levels of microplastics. 9Some studies in fish indicate that microplastic particles can cause oxidative damage to lipids in the gills and muscles, as well as neurotoxicity through the inhibition of acetylcholinesterase and alterations in neurotransmitter levels. 10mans, as an important component of the ecosystem, are also affected by plastic waste.It has been estimated that a weekly intake of microplastics (MPs) with values ranging between 0.1 and 5 g can be found bound to food and drinking water, thereby generating adverse health effects. 11In the city of Beijing, China, an analysis of feces conducted on young people between 18 to 25 years old who consumed water and food revealed the presence of microplastics such as polypropylene with a size of 20 -800 nm. 12 Another study in Mexico found up to 30 microplastic particles in a series of foods such as energy drinks, tea, sodas, and beers 13 ; another work conducted in Iran, in the analysis of bottled mineral water, found values of 8.5 AE 10.2 particles/L of PET, PS, PP. 14 MPs are a globally recognized problem due to their prevalence in natural environments and the food chain, as well as their high impact on human health.Plastics directly affect living beings, either through ingestion or toxicity.It is noted that they could act as vehicles for invasive species and by adsorption on their surface of other synthetic chemical pollutants such as polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), or organochlorines, currently used by the chemical industry, thus potentiating or synergizing their toxic power due to components they contain such as plasticizers, heavy metal additives, etc. 15 Studies on microbiota have allowed the assessment of the effect of MPs, especially PETs, on microbiota, demonstrating that they would act at the colon level, decreasing the values of Staphylococcus spp., Bifidobacterium spp., and Clostridium spp. 16VISED Amendments from Version 1 Based on the reviewers' comments, the relevant changes were made; hence, the changes made relate to: Paragraph 7 of the article was modified to indicate more precisely and specifically the different types that are used in the degradation process of LDPE plastics, including photo-oxidation, thermal degradation, ozonation, mechano-chemical degradation, catalytic degradation, and biodegradation.Paragraph 8 was separated from paragraph 7 to contextualize the importance of biodegradation and the action of microorganisms on polymers through different enzymes.
In the method section, the title of Table 1 was modified because the column containing the titles of the publications was excluded; on the other hand, a column equivalent to the sample used in the biodegradation was added; furthermore, the methods, microorganisms identified, and the analysis techniques were broken down into columns.
Any further responses from the reviewers can be found at the end of the article An important aspect to consider is the degradation process of plastics, such as with LDPE, which can take up to 400 years to decompose. 17Different types of degradation are used como son: (a) Mechanisms of photooxidation, this process uses light absorption, acting by photooxidation and photodegradation; (b) Thermal degradation, is carried out by depolymerization or accidental reaction, using initially high temperature and ultraviolet light; (c) Ozonation, the ozone present in the atmosphere causes the degradation of polymers, transforming them into so-called reactive oxygen species (ROS), which are a group of free radicals capable of producing oxidative damage; (d) Mechanochemical degradation, the process breaks the polymer chains by exposing them to mechanical stress and ultrasonic irradiation; (e) Catalytic degradation, residual polymers are catalytically transformed into hydrocarbons producing oils and gases; y, (f) Biodegradation, the process involves various microorganisms, mainly bacteria (aerobic or anaerobic) and fungi. 5e capability of hydroxylases, lipases, and laccases enzymes, secreted by LDPE-degrading microorganisms, which are responsible for breaking the polymer chain into low molecular weight fragments, must be mentioned. 18Extracellular enzymes play a very important role in biodegradation through the depolymerization of LDPE to form intermediate products that can be used as a carbon source by microorganisms, 19 as they oxidize, reduce, hydrolyze, esterify, and cut the internal molecular structure of the polymer. 20croorganisms accelerate and increase the degradation process, making them an alternative to reduce the accumulation of petroplastics in the environment. 21There are reports of bacteria (Pseudomonas spp.and Bacillus spp.) and fungi (Aspergillus spp.and Fusarium spp.) that can degrade this plastic under laboratory conditions. 22,23The use of more efficient microorganisms in the degradation of LDPE will allow the proper selection of bacteria or fungi with greater action and degradative efficiency of plastic. 24At the industrial level, it will involve the handling of various effective methods of detection and quantification, such as gravimetry, scanning electron microscopy (SEM), [25][26][27][28][29][30] Fourier transform infrared spectroscopy (FTIR), and gas chromatography coupled with mass spectrometry (GC-MS), which complement the study of the polymer's natural degradation. 31e purpose of this systematic review is to demonstrate the efficiency of LDPE-degrading microorganisms and the efficacy of the main biodegradation techniques on this type of plastics.

Methods
The PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) methodology was used, which is established for systematic reviews and meta-analysis statements. 32The information was extracted from articles obtained from various databases such as: Scopus, Web of Science (WOS), Embase, and Google Scholar.The identification, screening, and eligibility of scientific articles were organized through the Zotero Bibliographic Manager. 33The protocol of the systematic review was registered in PROSPERO (International prospective register of systematic reviews) under the number CRD42024506168.The advanced search in this database included the exact phrase "LDPE biodegradation"; at least one of the following terms: Fungi, bacteria, or microbial consortia; terms mentioned in all scientific articles.It was also possible to delimit the years and languages of publication for each study.

Selection criteria Inclusion criteria
• Scientific articles that included bacteria, fungi, and microbial consortia reported as LDPE degraders.
• Scientific articles that report the percentage of weight loss after the process.
• Articles published from January 2010 to October 2022.
• Publications in Spanish and English with open access.

Exclusion criteria
• Studies that do not report gravimetry.
• Genus or species of the polyethylene-degrading microorganism.
The coordination and development of the review activities were carried out through the Zoom video chat software.
To include the studies, their relationship with the research question was verified, based fundamentally on the terms: Biodegradation, LDPE, bacteria, and fungi.Then, for the quality assessment of the Systematic Reviews, Meta-analysis and a scientific article evaluation scale were used to ensure strict compliance with the inclusion and exclusion criteria mentioned in previous paragraphs.The web application used throughout the process of identification, selection, eligibility, and inclusion was Zotero.To collect relevant data from each report, PRISMA 2020 was used.Empirical articles were evaluated using an analytical rubric designed according to the parameters founded on the SSAHS scale by López-López E, Tobón S, JuárezHernández LG) for the consideration of scientific articles.Systematic reviews were evaluated using the Quality Assessment of Systematic Reviews and Meta-Analyses, utilizing an observation guide (checklist style as indicated by the National Heart, Lung, and Blood Institute). 34Data systematization tables were used considering year, author(s), sample, study type, methods, identified microorganism, and the study technique (TE) were included.The variables for which relevant information was sought were: degradation efficiency (DE) of LDPE degrading microorganisms, biodegradation of plastics, degradation time (DT), and percentage of weight loss.

Results
Figure 1 shows the PRISMA methodology, whose search protocol identified 483 primary articles, of which 133 duplicates, 279 by title, 5 by abstract, 11 by access, and 20 for not meeting the inclusion terms were excluded.A total of 35 fulltext articles were obtained, and 15 were included from previous review.
Table 1 indicates the number of articles found, totaling 50, with the highest quantity produced between the years 2018 to 2022.Also, it is observed that 100% of the articles correspond to experimental research works.Likewise, it points out the study techniques, with the most frequent being: SEM, gravimetry, and FTIR.Regarding the microorganisms identified in each of the studies, the most frequent in the phylum bacteria belonged to the genera Pseudomonas and Bacillus; as for the phylum of fungi, Aspergillus spp.predominated.As for the isolation site, the most used and mentioned in the reviewed articles were landfill soil and sanitary landfill. 32able 2 allows for the descriptive observation of microorganisms classified into bacteria, fungi, and microbial consortia, in quantities of 23, 17, and 9 respectively.Also, it specifies the species and the efficiency of LDPE-degrading microorganisms expressed in weight loss from highest to lowest and the days used for complete degradation, highlighting among bacteria Enterobacter spp., Pantoea spp., P. spp., Escherichia coli, and B. spp.which obtained an ED range of 9.00-70.00%,24.00-64.00%,

Discussion
According to the data analyzed (Table 1), in recent years there has been an increase in studies on LDPE-degrading microorganisms with the aim of minimizing environmental impacts through bioremediation. 78The requirement for special technologies allows understanding the degree of polymer disintegration and the nature of its resulting products.In the SR, up to 20 study techniques used in the biodegradation of LDPE have been detected.From our point of view, we consider highlighting in this article those methods that, due to frequency and especially efficacy, stand out among others.These methods include the following: SEM, 67,69-71 gravimetry, 25,26,27,28,29,30,35,36,39,40 and FTIR. 20,28,60,66 Pseudomonas spp. 19,20,25,28,30,36,39,41,50,59,68The SEM study technique, 19,[42][43][44]46,[49][50][51][52][53][54]56,62,63,70,72 is used to detect the biodegradation of LDPE and is employed to monitor changes on the surface of the LDPE film. 72,[74][75][76] The adhsion of microorganisms to the surface is essential for biodegradation.65 After incubating LDPE with selected degrading microorganisms on the surface, some characteristics such as erosion, holes, and cavities are observed, which are attributed jointly to the formation of bacterial film and the penetration of fungal hyphae.60,68 Erosion is considered the primary cause of the mass reduction of the surface due to the secretion of enzymes and microbial extracellular metabolites.20 Another frequently used technique in the reviewed articles is gravimetry, 20,[42][43][44][45][46][48][49][50][51][52][53][54][55][68][69][70][71] which is a simple and highly precise test to determine the polymer weight reduction, originating as a consequence of being used as a source of carbon and energy by microorganisms.19,[62][63][64][65][72][73][74]76 This weight loss is considered proportional to the surface area, as biodegradation starts on the polymer's surface.38 With Gram-positive bacteria, the degradation efficiency (DE) in terms of LDPE weight loss has been reported: Streptomyces sp.DE 5.2% and degradation time (DT) of 90 days 40 and Bacillus amyloliquefaciens DE 11% and DT 60 days.38 It was found in the review that various articles mentioned the FTIR technique, 19,25,26,27,28,29,30,35,37,40,44,46,50,51,64,[67][68][69][70][74][75][76] as the third most frequent of those applied to determine biodegradation; in addition, the cited studies consider it analytical and efficient, useful for identifying the chemical configuration of organic, polymeric, and inorganic material, and the morphological changes, which are supported by the chemical structural changes at the level of the carbon chains, observing new functional groups (alkoxy, acyl, carboxyls, and nitro) or absence of them, and modifications in the chains such as breaks, stretches, and formation of double bonds; moreover, this technique determines the carbonyl index (CI), which measures the degree of degradation of the LDPE and in which its value depends on the degraded carbonyl bonds.28 In reality, it involves measuring the concentration of carbonyl groups (CG) corresponding to acids, aldehydes, and ketones. 35 In th process of LDPE biodegradation, the initial weight corresponds to the oxidation of the chain that leads to the formation of CG, and subsequently, these form carboxylic groups that are degraded by β-oxidation and then through the citric acid cycle to CO 2 and H 2 O. 77 In Table 2, as observed, the microorganisms frequently reported in the articles analyzed in Table 1 include bacteria from the genera Bacillus, Brevibacillus, Cellulosimicrobium, Comamonas, Delftia, Enterobacter, Escherichia, Idonella, Kocuria, Lysinibacillus, Paenibacillus, Pantoea, Pseudomonas, Rhodococcus, Rhodotorula, Stenotrophomonas, and Streptomyces, 25,28,30,42,46,58,59 with the genera Pseudomonas, Bacillus, and Streptomyces predominating.Other microorganisms are fungi, with Aspergillus sp.48,50,52,63 Among the less frequently cited species include Rhizopus oryzae, Paenibacillus sp., Streptomyces coelicoflavus, Thiobacillus, Clostridium, Achromobacter denitrificans, Penicillium oxalicum, P. chrysogenum, Pycnoporus sanguineus, Enterobacter cloacae.29,37,44,45,54,55,61,74 In the same table, it is analyzed that among the most efficient bacteria in the degradation of LDPE according to the weight loss of the polymer include several species such as Enterobacter spp. with an ED of 9.00 -70.00%, and TD of 4 -150, Pantoea spp. with an ED of 24.00 -64.00% and TD of 120, Pseudomonas spp. with an ED of 1.15 -61.00% and TD of 4-150, Escherichia coli with an ED of 45.00% and TD of 30, and finally, Bacillus spp. with an ED of 1.50 -40.00% and TD of 30-120.Bacillus sp. is also considered as another important species in the biodegradation process, having a consumption rate of 0.0019 g of the polymer per day 45 ; or, participating in consortia such as the one constituted by Bacillus vallismortis, Pseudomonas protegens, Stenotrophomonas sp., and Paenibacillus sp.42 It has been determined that P. aeruginosa cultured on LDPE as the only carbon source has an ED of 0.0015 g of LDPE per day and a TD of 462 days to reduce a polyethylene film from 1g to 0.5g 20 ; Enterobacter cloacae AKS7 and Escherichia coli possess another type of degradative action, and it is due to the secretion of extracellular polymeric substances and the high hydrophobicity of the microorganism's cell wall, which allows a greater formation and adherence of the bacterial biofilm.36,61 In the case of Pantoea sp., its efficiency can be measured either individually or in consortium with Enterobacter.41 Fungi (Table 2), like bacteria, are considered LDPE-degrading microorganisms.The most efficient are: Neopestalotiopsis phangngaensis, Colletotrichum fructicola, and Thyrostroma jaczewskii with EDs of 54.34, 48.78, and 46.34%, respectively, and a TD of 90 days.The mycotic activity is considered to be due to their great capacity for adherence.In the polymer biodegraded for 40 days, the biofilm formed by the strongly adhered fungi is observed; at 80 days, surface deformation is evident and microcracks are differentiated.28 Other efficient species are also reported, such as A. clavatus with an ED of 35.00% and a TD of 90 days 27 and A. versicolor with 40.60% and a TD of 90 days.43 The degradative efficiency of microbial consortia has also been reported in different studies, 35,41,63,70 showing the most efficient to be the one formed by Enterobacter spp.and Pantoea sp., and the one of Pseudomonas protegens, Stenotrophomonas sp., B. vallismortis, and Paenibacillus sp. The coperation of different microorganisms allows the use of different and complementary metabolic capacities for their growth, forming pure or mixed biofilms (fungi and bacteria), more resistant and metabolically more active.79 LDPE-degrading microorganisms form a biofilm on the polymer and use it as a carbon source for consumption, an event that is reflected in weight loss.The biodegradation by microorganisms is a process of high metabolic activity, in which the count of viable cells, the concentration of surface proteins, and the efficiency in degradation expressed as polymer weight loss must be taken into account.20 The most frequent place of isolation of microorganisms with LDPE-degrading capacity came from landfill soil and sanitary landfills with the presence of plastics.Various studies have indicated that bacteria and fungi adapt under different environmental conditions, a process mediated by complex cellular changes at the enzymatic level, 19,56 maintain physiology and metabolism, thus ensuring the survival of microorganisms.It has been demonstrated that bacteria such as Pseudomonas putida is a resistant and efficient xenobiotic decomposer because it presents an effective efflux pump; similarly, Streptomyces atacamensis shows xerotolerant latency and spore response to desiccation, and upregulation of proteins that are functional during xeric stress, 80 which probably explains why certain microorganisms are more efficient at degrading LDPE compared to others.
Considering that degradation is a slow process (this activity occurs before 60 days of incubation), and that degradation methods are heterogeneous, some authors conclude that there is no standard methodology in relation to analytical methods. 15However, in this article, we present the various biodegradation techniques, so a more precise vision could be had to assess which of them is the most consistent and effective according to their ED and TD.
Finally, based on the detection and quantification tests of polymer degradation, the exposed microorganisms constitute a sustainable alternative, useful for bioremediation and minimization of environmental impacts, with the aim of reducing environmental pollution by LDPE.• The most effective techniques used in LDPE biodegradation are SEM, gravimetry, and FTIR.

Limitations
The results obtained allow for the identification of a lack of studies on microorganisms efficient in the biodegradation of LDPE, which limits the possibility of expanding their number and understanding their efficiency.Moreover, there are few studies on alternative methods that are effective in biodegradation.These limitations should be taken into account for the guidance and development of new research.
In the introduction, it is necessary to include a more detailed paragraph on the plastic degradation techniques such as (thermal, radiation, mechanical, chemical and biological), then put emphasis on the biological ones, indicating the advantages and limitations in the biodegradation of lowdensity plastic, to have a clearer picture of this review, then eliminate the research question rather place the implication or impact that this work has.
Regarding the results, consider in Table 1 to consider the classification as follows: (Year, Authors, Type of study, type of sample or sample, Technique (physical, mechanical or biological), identified microorganisms, Analysis in the detection of Degradation.
The discussion needs to go deeper into Table 1.

Are sufficient details of the methods and analysis provided to allow replication by others? Yes
Is the statistical analysis and its interpretation appropriate?I cannot comment.A qualified statistician is required.

Are the conclusions drawn adequately supported by the results presented in the review?
contamination and the effects on human health and wildlife.The results of this study provide a comprehensive view of the biodegradative capabilities of different microorganisms and effective methodologies for evaluating plastic degradation, which can guide future research and industrial applications in plastic waste management.

Conceptualization of Biodegradation Techniques:
Comment: It would be beneficial for the introduction of the study to include a detailed conceptualization of different biodegradation techniques, covering aerobic and anaerobic processes, necessary environmental conditions, types of microorganisms involved, and specific applications for LDPE plastics.This would help readers understand how these techniques are applied and evaluated in the context of plastic biodegradation, providing a clear and complete theoretical framework from the outset.

Diversity of Evaluation Techniques:
Comment: The study highlights several evaluation techniques such as SEM, gravimetry, and FTIR but would benefit from a more in-depth discussion of the specific advantages and limitations of each technique.Rationale: A detailed comparison would help readers choose the most suitable techniques for their own research and practical applications.

Future Research in Microbial Consortia:
Comment: The review mentions the effectiveness of microbial consortia in LDPE degradation but could be expanded to suggest additional studies exploring the synergistic interaction between different microorganisms.Understanding how microbial consortia interact and enhance the degradation of plastics can open new avenues for research and development of more efficient biotechnologies.

Environmental and Human Health Impact:
Comment: Although the impact of plastics on the environment and human health is addressed, a section specifically dedicated to discussing these effects with recent data and concrete examples would strengthen the study's argument.Providing detailed information on the environmental and public health consequences of plastic accumulation underscores the importance of developing and applying effective biodegradation techniques.
The article offers a comprehensive and detailed overview of the biodegradative capabilities of various microorganisms and the effective methodologies for the degradation of LDPE.However, it could benefit from further conceptualization of biodegradation techniques and a more detailed explanation of the inclusion and exclusion criteria.Additionally, expanding the discussion on the advantages and limitations of evaluation techniques, synergy in microbial consortia, and the environmental and human health impacts would provide a more complete and applicable perspective.
Are the rationale for, and objectives of, the Systematic Review clearly stated?Yes

Are sufficient details of the methods and analysis provided to allow replication by others? Yes
Is the statistical analysis and its interpretation appropriate?I cannot comment.A qualified statistician is required.

Are the conclusions drawn adequately supported by the results presented in the review? Yes
If this is a Living Systematic Review, is the 'living' method appropriate and is the search schedule clearly defined and justified?('Living Systematic Review' or a variation of this term should be included in the title.)Yes Competing Interests: No competing interests were disclosed.
Reviewer Expertise: Bioremediation and Biodegradation of organic contaminants I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.
The benefits of publishing with F1000Research: Your article is published within days, with no editorial bias • You can publish traditional articles, null/negative results, case reports, data notes and more • The peer review process is transparent and collaborative • Your article is indexed in PubMed after passing peer review • Dedicated customer support at every stage • For pre-submission enquiries, contact research@f1000.com

Table 1 .
Articles Classified by Year, Author, Study Type, Sample, Methods, Identified microorganisms and Study Technique.